Algoriphagus machipongonensis

Algoriphagus machipongonensis strain PR1 genome: https://www.ncbi.nlm.nih.gov/assembly/GCF_000166275.1

The A. machipongonensis assembly was created from a number of data sets

• Illumina data: http://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR032989
• Sanger sequencing from JVCI: ftp://ftp-private.ncbi.nlm.nih.gov/pub/TraceDB/algoriphagus_sp__pr1/
• Independent assembly of reads from the whole genome sequencing of the S. rosetta genome.  to pull out the Algoriphagus sequences, align to the reference sequence
  • ftp://ftp-private.ncbi.nlm.nih.gov/pub/TraceDB/proterospongia_sp__atcc_50818/ 
  • Sanger fosmids (454 paired and fragment): http://www.ncbi.nlm.nih.gov/Traces/study/?acc=SRP02276  

Genomes of other Algoriphagus species: https://www.ncbi.nlm.nih.gov/assembly/?term=algoriphagus%20genome

Choanoflagellates

Learn more about choanoflagellates!

• ChoanoWiki (the worldwide choanoflagellate community)
• Tree of Life description of choanoflagellates

Reagents

• Obtain choanoflagellates strains from ATCC
• Salpingoeca rosetta ATCC 50818 (formerly known as Proterospongia sp.)
• Monosiga brevicollis ATCC 50154
• Order cDNA libraries from Addgene

Growing choanoflagellates

• Making culture medium
• Thawing choanoflagellates from frozen stock
• Passaging choanoflagellates
• Long-term storage of choanoflagellates

Choanoflagellates genomes

• S. rosetta genome browser (Broad Institute/MIT)
• M. brevicollis genome browser (Joint Genome Institute)